Induction of Gene Expression Research Paper Example | Topics and Well Written Essays - 1250 words

Induction of Gene Expression - Research Paper Example They are preceded by a single promoter/operator region which controls the expression of all three genes. Even further upstream of the promoter lies the gene lacI which codes for the lac repressor protein. This protein is a regulator and binds to the promoter/operator region of the lac operon in the absence of lactose in the medium. This is simply an economic measure by the bacterium to prevent the wasteful synthesis of enzymes when they are not needed. In the presence of lactose, the repression is relieved as lactose binds to the repressor protein and changes its conformation in a manner that makes it dissociate from the promoter. However, there is an added control level to the regulation of this operon. In the presence of a preferred substrate, like glucose or its modified form, glucose -6- phosphate, the bacterium will still not synthesize lactose even though this is present in the medium along with glucose. This phenomenon is called catabolite repression. The mechanism involves the CAP protein which also can increase expression of the lac operon. When glucose levels are high, cyclic AMP levels lower. Cyclic AMP forms a complex with CAP before it binds to the DNA. So, when the cyclic AMP levels are lowered, the CAP protein bound to DNA also decreases, thus lowering the transcription of lac genes. ... Since the natural substrate lactose and the products of its metabolism are not coloured detection of their formation is difficult. For this purpose, the analog ONPG is used which upon hydrolysis yields a product which is deep yellow in colour and can be spectrophotometrically quantified to follow the reaction and hence the expression patterns of the operon. The aim of the work is to use this analog and others to obtain a better understanding of the workings of the lac operon. MATERIALS AND METHODS Culturing of the bacteria: 10 ml each of E. coli (lac+ strain) which had reached mid-log phase was aliquoted into separate flasks and incubated at 28- 30 C gently shaking to ensure aeration. The cells were allowed to continue growing. Induction: Two sets of induction experiments were performed. The first set was induced with IPTG at a final concentration of 0.5 mM. The second set was also induced with 0.5 mM IPTG but in addition glucose was added to the medium to a final concentration of 30 mM. For the first set 1 ml samples were taken out at intervals of 2, 4, 8, 16, 24, 32 and 48 minutes and used for the assay of galactosidase activity. For set 2, samples were removed at intervals of 10 and 45 minutes after induction with IPTG. As a control, 1 ml of the culture was removed prior to induction from both sets and used as uninduced controls. -galactosidase assays: To determine whether expression of the operon was taking place, the activity of galactosidase was assayed as follows. 0.1 ml of the culture samples removed at each time point were transferred into spectrophotometer tubes appropriately labeled. 1.5 ml of ONPG assay medium was added to each tube. (100 ml of assay medium contains 8 mg ONPG, 0.1 ml mercaptoethanol, 0.001 M MgSO4, pH7). After brief vortexing

Okonkwo Essay Example for Free

Okonkwo Essay Some regard the arts as having an extra-artistic cognitive function, that they have a message about mans place in the world which might have social or political implications. For example, there might be a case for supposing that the arts have an important function as a medium for social criticism and transformation. In any case, there is a widespread belief that the arts have a higher purpose to educate by encouraging introspection and sometimes making us think about how we should live our lives. Two heads are better than one. Learning nd working in groups involves shared or learned values, resources, and ways of doing things. Group projects can reinforce skills that are relevant to both group and individual work, including the ability to: break task into parts, plan and manage time and gain communication skills. What strategies were used? Many of my classmates including myself would have preferred to work on this project individually, however every now and then more hands make for lighter work. When my partner and I got together to begin our project we had decided to split the project in half. Same an equal amount of work. I wouldnt say we used and specific strategies to begin the Mexican Revolution project. We did however follow the some of the guidelines of the design cycle to begin which included: plan, investigate, design and create. What knowledge was acquired? Because my partner had so much knowledge of the Mexican Revolution, she was able to help me clearly understand/clarify certain things that had occurred that I was uncertain of. Because we had to do some research on our own I gained more on the revolution, to the porfiriato era where porfito Diaz himself introduced railroads, to ow important the constitution was for the people. What skills were utilized and developed? Group projects can also help students develop skills to collaborative efforts, allowing students such as myself to: delegate roles and responsibilities, share perspectives, knowledge and skills, hold one another and be held accountable. (We were able to use pervious knowledge from class and what we have been taught to be able to complete the project. I was able to develop and gain more knowledge of the Mexican revolution because of my partners knowledge. ) What will be done ditterently in the tuture? A lot of things will be done differently in the future. Time management of course. We ended up doing it the same week it was due which caused some stress on each of us individually. Although we completed out project in time I would have liked to have started planning the week she told us Just to have had a head start and know what we were doing then actually doing it last minute and not really knowing what we were going to do. Even if we decided to split the work, I would have wanted to still worked together since it was a group project. But above all, it was a great experience working with another individual. 378

The Villain In A Separate Peace English Literature Essay

The Villain In A Separate Peace English Literature Essay In the novel, A Separate Peace, the main villain is Gene Forrester. One reason Gene is the villain is because Genes thoughts and views are envious. Ellis says that Gene is not capable of maintaining Phineass spiritual purity and, as a result, Gene is cruel to Phineas. Knowles informs that Gene assumes that there is no harm to envy your best friend since Gene thinks envying Phineas is perfectly normal. Another reason Gene is the villain is because Phineass death involves Gene and his cruelty. Ellis explains how Gene discovers his private evil, which causes him to hurt Phineas. Knowles describes how Gene already ends his own war, given that he kills his enemy at Devon School. Gene is the villain because his thoughts and views are envious. In the article it says, The tragedy of the novel ultimately is that Gene is not capable of maintaining the spiritual purity that distinguishes Phineas and so must as he discovers his own savagery betray Phineas  (Ellis 314). This quote explains that Gene finds his own odium towards Phineass spiritual personality. Therefore, Gene wants to be better and more superior than Phineas, causing him to be jealous of Phineas. As Gene becomes jealous, he gains savagery against Phineas because he cannot maintain the perfect image of his best friend. Gene also betrays Phineas and their friendship because Gene holds a grudge against his best friend. In conclusion, Gene discovers envious thoughts in which ends irritates Phineass presence. The amount of hatred and greed Gene has against Phineas symbolizes a villains personality. The amount of greed that Gene carries toward Phineas depicts him as a villain. In the novel, it states, I couldnt help envying him that a little, which was perfectly normal. There was no harm in envying even your best friend a little (Knowles 25). This quote explains that Gene starts to feel greed and soon envies his best friend, Phineas. Gene begins to act paranoid about being competitive with Phineas and, as a result, Gene becomes very jealous and alert. As Gene starts to envy his best friend, it shows how untrustworthy and evil Gene can be. It also shows that Gene admits that he does envy his best friend, Phineas, but it takes him a while to finally admit to himself that he feels this sense of jealousy. Gene just explains it in a way so that his thought of Phineas seems normal and uses it as an excuse so that it seems normal and ordinary. These cruel traits that Gene upholds can be categorize in the qualities of a true villain. Another reason Gene is the villain is because Phineass death involves Gene. Ellis states, Gene has discovered that his private evil, which caused him to hurt Phineas, is the same evil only magnified that results the war (318) This quote explains that Gene has an amount of evil within himself and it causes him to hurt Phineas. Gene shows no self control and an adolescent mind since he hurt his best friend due to jealousy. In addition, Phineass death involves Gene because he has so much evil and cruelty to be motivated to jounce the trees limp causing Phineas to fall which led to the beginning of his downfall. If Gene never discovered this internal evil then Phineass life would have never be on risk. Genes evil plays a huge role in classifying him as a true villain because his intense level of hate makes him infuriate about Phineass accomplishments and actions. Genes involvement in Finnys death classifies him as a villain. In the novel, it says, I never killed anyone and I never developed an intense level of hatred for the enemy. Because my war ended before I ever put on a uniform; I was on active duty all my time at school; I killed my enemy there (Knowles 204). This quote explains that Genes involvement in Phineass death was his goal or desire. At Deven, Gene wanted to end Phineass life so that he can look superior and will not have to worry about someone being better than him anymore. This quote also shows the huge amount of hatred and the lack of loyalty Gene has toward Phineas because Gene just wants to finish his own war. He results the end of his war by ending his best friends life and he does success since he takes out with bothered him the most. Finally, the quote illustrates that Gene shows no pity since he did not care as much and Gene also shows any sadness about Phineass death. Similar to a true villain, Gene terminates his ene my by the amount of hatred and envious desires. Therefore, Gene ends his war at Devon School by Phineass death and he was able to accomplish this by the intense level of hatred. Gene Forrester is the true villain in the novel, The Separate Peace. Genes envious thoughts and views make him develop an amount of hatred and greed towards his best friend, Phineas. Gene even finds his odium against Phineass personality, which soon makes him discover his savagery against Phineas. His envious thoughts make him envy his best friend and. as a result, increases the amount of greed and tension between both of them. The involvement in causing Phineass death also clarifies why Gene is the true villain. Genes evil causes him to make a goal, in which it involves the end of Phineass life so that his own war would end. Gene shows a variety of qualities and characteristic in which clarifies why Gene is the true villain in this novel.

Bram Stokers Dracula: A Struggle to Maintain Victorian Upper and Middl

The Victorian men and women conveyed in Bram Stoker's Dracula are pure and virtuous members of the upper and middle class. However, hiding behind this composed and civilized conception of England lies a dark and turbulent underbelly. This underbelly is the lumpenproletariat, whom Karl Marx defined as "the lowest and most degraded section of the proletariat; the ‘down and outs’ who make no contribution to the workers cause". Victorian culture discriminated against these vagrants, who were seen not only as shiftless and immoral, but dangerous as well. Sex was taboo and purity was held sacred to the Victorian middle and upper class, but prostitution and sexually transmitted diseases ran rampant among the lumpenproletariat. The rich strive to be pious and good, but consider those of lower social standing to be less than human. The reaction of the characters in Dracula to the evil of the vampires can be likened to the Victorian conception of the lower classes. They were seen as a hedonistic but powerful force, with the collective capacity to end the affluent citizen's way of life. In this sense, the novel can be viewed as a struggle to maintain upper-class Victorian traditions against the traditions of the lower class. This paper will examine the similarities between the vampires and the perception of the lower classes in regards to superstition, sexuality, inequality and the "preying" of the lumpenproletariat on the respectable middle-class. It will also examine the signs evident in the novel of the Victorian mindset. Dracula is an aristocrat with a castle and noble title, but in reality he is more associated with the lumpen. While trapped in Dracula's castle early in the novel, Jonathan discovers that he has no hired help, has been performing menial tasks such as bed-making and table setting in secret, and even acting as the horse-carriage driver. He slumbers in dirt, much like the homeless, and is nomadic for most of the book. The Count associates himself most clearly with the lumpenproletariat in the form of a horde of gypsies who "attach themselves as a rule to some great noble"(49). They even do his bidding: "The [gypsies] have given me these"(50) Dracula says of Jonathan's ill-fated letters. Dracula attempts to hide his lumpen nature and exude an aristocra... ... "stagnant and foul" air (111). Vampires and the poor are projected as animal-like, and both prey on others. Since the vampires in Dracula can be seen as a representation of the lower class, we can draw some assumptions. If one draws this parallel to its logical conclusion, the battle with the vampires, it can be seen that in the end the lower class and their perceived values are soundly defeated by the righteous ruling class, and the threat is expelled from Britain. However, this ruling class does not come out of the battle unscathed, and the Victorian tradition is mostly lost. By reading Dracula as a battle to maintain Victorian tradition, one can more fully understand the actions of the characters. Their prudishness, Seward's scientific adherence to logic, and the idea of the fall from grace take on a new meaning in the light of such an understanding. Just as the characters in Dracula cling to their humanity, so too do they cling to their upper-class values in the face of the lower class. Works Cited Stoker, Bram. Dracula. England: Pengiun, 2003. defn:"Lumpenproletariat". Oxford English Dictionary. http://dictionary.oed.com

Cassius Analysis

Edgar KarapetyanKarapetyan 1 1 October 2012 Cassius vs. Rome Character Analysis Essay â€Å"The first method for estimating the intelligence of a ruler is to look at the men he has around him. † In William Shakespeare’s â€Å"The Tragedy Of Julius Caesar†, there are many dangerous people who are around Caesar. Cassius, supposedly one of Caesars close friends, devises a group called the Conspirators to end Caesars time of rule. This play shows how they kill him, and how Rome goes through chaos as a result.Cassius is a fascinating character created by Shakespeare. Although Antony shows many characteristics of an intelligent person, Cassius is the smartest character in this play because he has more admirable traits. A great trait to have as an individual is the power to manipulate people into doing whatever you want. Fortunately, Cassius has that ability. After devising a plan to assassinate Caesar, he searched for a particular group of people who were powerful and had the same wish he had. The most important person for his team was Brutus. The fault, dear Brutus, is not in our stars, but in ourselves, that we are underlings†(28) is what Cassius tells Brutus to try and pursue him to join the conspirators. He said that Caesars rise is their fault because they are not doing anything to stop it. Cassius used his smart, clever, and manipulative ways to tell Brutus that basically, you are helping Rome by killing Caesar. â€Å"And since you know you cannot see yourself/ so well as by reflection, I, your glass/ Will modestly discover to yourself†(25) is said to Brutus to persuade him to join the conspiracy because Cassius will help him find himself.It is this manipulative ability that allows Cassius to carry out his plan and, ultimately, kill Caesar. Imagine how difficult it would be to kill a ruler. It is practically impossible unless you have the mind of a genius and the sharpness of a killer. Thanks to Cassius’ clever mind and precise planning, he accomplished this goal. Even though he ended up killing himself, he successfully completed his mission. . â€Å"He reads much;/He is a great observer, and he looks/ Quite through the deeds of men†(30-31) is said by Julius Caesar to describe Cassius. This shows that even though Julius Caesar is a stubborn, selfish person, he sees Cassius’ power.Cassius was like Michael Jordan in the 1991 NBA Finals. He was committed to succeeding his mission no matter what it took. â€Å"Yond' Cassius has a lean and hungry look; He thinks too much: such men are dangerous†(30). Based on what Caesar said about Cassius, this shows that he is so focused, other people can easily see it in his face. Cassius used his skills for the wrong reasons, but you can definitely see what kind of person he really is. The flaw that Cassius has that is very easily seen throughout this play is his jealousy of Caesar. Cassius wants the people to look at him the way they look at Ca esar. ‘But in ourselves, that we are underlings. Brutus and Caesar: what should be in that ’Caesar’? Why should that name be sounded more than yours? ’ (28). By trying to kill him, he thought that they would see him and Brutus as their saviors, but, instead, they saw them as traitors of Rome due to Antony’s words. â€Å"I was born free as Caesar; so were you: / we both have fed as well, and we can both / Endure the winter's cold as well as he†(27) is what Cassius says about Caesar. Cassius believes that he is just as great as Caesar and does not think that Caesar should be treated specially.Cassius’ eye for power is what causes him to kill himself. He saw that his plan had gone too far and that he would be captured so he committed suicide. Unlike Cassius, Brutus had killed Caesar for the love of Rome. Cassius is truly the villain in this story by Shakespeare. â€Å"The only thing necessary for the triumph of villains is for good men t o do nothing† Edmund burke. In William Shakespeare’s The Tragedy Of Julius Caesar, the good men of Rome do not allow the villain, which in this case is Cassius, to go without being punished.They pursue him until he is forced to commit suicide. Among these good people, there is Octavius, Lepidus, and most importantly, Antony. Sometimes, the smartest character is not necessarily the good guy. Through his cleverness, manipulative ability and hate for Caesar, Cassius accomplished what was practically impossible†¦ to kill a God. Works Cited â€Å"Notable Quotes in Julius Caesar. † Notable Quotes in Julius Caesar. N. p. , n. d. Web. 08 Oct. 2012. ;http://www. shakespeare-navigators. com/JC_Navigator/notable_quotes. html;.

Joint Venture Essay

Joint ventures are business ventures formed by two or more companies to achieve aspecific, but limited, objective. An example would be the development of an offshore oil field, where a group of companies combines to build and operate a drilling platform and related pipeline. The project is owned equally by the affiliated enterprises and its management could be controlled either by one of the partners or by a separate management could be controlled either by one of the partners or by a separate management organisation established just for the project. The venture may be organised as a partnership ,a corporation ,a joint-stock company or some other legal form, and it can contunieindefinetly.The rational for a joint venture is usually to diversify risk among the members and or to combine expertise and assets that none of the participants provide alone. : Roy L.Crum,Eugene F.Brigham& joel.F.Houston.(2005)Fundamentals of international finance.1st edn. THE ADVANTAGES OF JOINT VENTURES: * Access to expertise and contacts in local markets, each partner agrees to ajoint venture to gain access to the other partner’s skills and resources. Typically the international partner contributes financial resources, technology or products. The local partner provides the skills and knowledge required for managing a business in its country. * Reduced market and political risks * Economies of scale by pooling skills and resources(resulting in e.g. lower marketing costs) * May avoid local tariffs and non-tariffs barriers * Shared risk of failure * Possibly better relations with national governments through having a local partner. THE DISDVANTAGE OF JOINT VENTURES: * Objectives of the respective partners may be incompatible ,resulting in conflicts * Contributions to joint ventures can become disproportionate * Los of control over foreign operations * Completion might overburden a company’s staff * Partners may become locked into long term investments from which it is difficult to withdraw * Cultural differences may result in possible differences in management culture among participating firms * Problems of management structures and dual parent staffing of joint ventures. * Transfer pricing problems as good pass between partners Sven hollensen.(2004)global marketing(adecision-oriented approach).3rd edn.